Meyer, A. J., Segall-Shapiro, T. H., Glassey, E., Zhang, J. & Voigt, C. A. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat. Chem. Biol. 15, 196–204 (2019).

In this publication, Meyer and colleagues characterize fourteen inducible promoters to be used as inputs to a circuit, twelve of which have shown desirable properties after directed evolution. Other characteristics have been calculated for the parts, such as orthogonality, leakiness, a time-course analysis, toxicity, evolutionary stability and their effectiveness while inserted in the genome. The strain E. coli Marionette was built to optimize the use of all Input promoters in the same cell.

The output of each promoter is calculated in relative promoter units (activity relative to BBa_J23101), which allows comparation with some other parts in this repository.