CRISPR Systems

Tools for multiplexed and orthogonal CRISPR gene regulation.

The breadth of tools using CRISPR associated (Cas) proteins is larger than the scpe of this database. Here only tools for simple CRISPRi (interference/repression) and CRISPRa (activation) are presented. Although only simple Dynamic ranges and high/low expression levels are presented in this table, many more properties of these systems were described, as showed on specific parts pages.

Apart from the Cas9 variants collection, the other two libraries were measured using the Streptococcus pyrogenes dCas9. The repression system (ELSAs) is based on the regulation of σ factor 70-activated promoters and the activation system is based on regulation of σ factor 54-regulated promoters.

Show quantitative parameters:

Dynamic Range
High/Low Expresion